主要科研领域与方向
　　重要信号蛋白的泛素化降解调控；
　　STAT3蛋白活性调控；
　　TGF-β，Wnt，FGF等信号在细胞增殖与分化中的作用；
　　肿瘤转移的基因调控；
　　IL-17在炎症反应中的作用。

　Selected Publications
　·Yu Shang, Xinghui Zhao, Xialian Xu, Hong Xin, Xueni Li, Yonggong Zhai, Dacheng He, Baoqing Jia, Wei Chen, and Zhijie Chang*. CHIP functions as an E3 ubiquitin ligase of Runx1. Bioch. Bioph. Res. Com. 386:242-246, 2009.
　·Zhen Qin, Fangli Ren, Xialian Xu, Yongming Ren, Hongge Li, Yinyin Wang, Yonggong Zhai, and Zhijie Chang*. ZNF536, A Novel Zinc Finger Protein Specifically Expressed In The Brain, Negatively Regulates Neuron Differentiation Via Repressing RA-Induced Gene Transcription. Mol. Cell. Biol. 29:3633-43, 2009.
　·Zhili Rong, Andrew Wang, Zhiyong Li, Yongming Ren, Long Cheng, Yinghua Li, Yinyin Wang, Fangli Ren, Xiaoning Zhang, Jim Hu and Zhijie Chang*. IL-17RD (Sef or IL-17RLM) Interacts with IL-17 Receptor and Mediates IL-17 Signaling. Cell Research. 19:208-215, 2009.
　·Fang Wang, Haiwei Zhang, Xinjun Zhang, Yinyin Wang, Fangli Ren, Xiaoning Zhang, Yonggong Zhai*, and Zhijie Chang* Varp interacts with Rab38 and functions as its potential effector. Bioch. Bioph. Res. Com. 372:162-167, 2008.
　·Xueni Li, Mei Huang, Huiling Zheng, Yinyin Wang, Fangli Ren, Yu Shang, Yonggong Zhai, David M. Irwin, Yuguang Shi, Di Chen, and Zhijie Chang*. CHIP promotes Runx2 degradation and negatively regulates osteoblast differentiation. The Journal of Cell Biology. 181(6):959-972, 2008.
　·Yongming Ren, Long Cheng, Zhili Rong, Zhiyong Li, Yinghua Li, Xinjun Zhang, Shiqin Xiong, Jim Hu, Xin-Yuan Fu, Zhijie Chang*. hSef potentiates EGF-mediated MAPK signaling through affecting EGFR trafficking and degradation. Cellular Signalling 20:518-533, 2008.
　·Ren-Feng Li, Yu Shang, Di Liu, Ze-Song Ren, Zhijie Chang*, Sen-Fang Sui*. Differential Ubiquitination of Smad1 Mediated by CHIP: Implications in the Regulation of the Bone Morphogenetic Protein Signaling Pathway. Journal of Molecular Biology. 374:777-790, 2007. (co-corresponding authors).
　·Zhili Rong, Long Cheng, Yongming Ren, Zhiyong Li, Yinghua Li, Xueni Li, Hongge Li, Xin-Yuan Fu, Zhijie Chang*. Interleukin-17F Signaling Requires Ubiquitination of Interleukin-17 Receptor via TRAF6. Cellular Signalling 19:1514-1520, 2007.
　·Sen Liu Shiyong Liu, Aoneng Cao, Zhijie Chang, Luhua Lai*. Non-natural Protein-Protein Interaction-Pair Design: a PH Domain Designed to Bind with EPO Receptor by Key Residues Grafting. Proc. Natl. Acad. Sci. USA. Vol. 104(13): 5330-5335, 2007.
　·Yongming Ren, Zhiyong Li, Zhili Rong, Long Cheng, Yinghua Li, Zhen Wang, and Zhijie Chang* Tyrosine 330 in hSef is critical for the localization and the inhibitory effect on FGF signaling. Bioch. Bioph. Res. Com. Vol. 354(3), 741-746, 2007.
　·Zhijie Chang, Lorne A Babiuk, Jim Hu* Therapeutic and Prophylactic Potential of siRNAs against SARS: Progress to Date. BioDrugs (Invited review). Vol. 21(1):9-15, 2007.
　·Yongming Ren, Long Cheng, Zhili Rong, Zhiyong Li, Yinghua Li, Hongge Li, Zhen Wang, Zhijie Chang*. hSef Co-localizes and Interacts with Ras in the Inhibition of Ras/MAPK Signaling Pathway. Bioch. Bioph. Res. Com. Vol. 347(4), 988-993, 2006.
　·Zhili Rong, Yongming Ren, Long Cheng, Zhiyong Li, Yinghua Li, Yang Sun, Hongge Li, Shiqin Xiong, and Zhijie Chang* Sef-S, an alternative splice isoform of sef gene, inhibits NIH3T3 cell proliferation via a mitogen-activated protein kinases p42 and p44 (ERK1/2)-independent mechanism. Cellular Signalling 2007 Jan; 19(1):93-102.
　·Yu Rong, Long Cheng, Hongxiu Ning, Jizhen Zou, Yuanjiang Zhang, Fang Xu, Li Liu, Zhijie Chang*, and Xin-Yuan Fu* WT1 and STAT3 Synergistically Promote Cell Proliferation: A Possible Mechanism in Sporadic Wilms' Tumor. Cancer Research. 2006 Aug 15;66(16):8049-57. (co-corresponding authors)
　·Xinjun Zhang, Xi He, Xin-Yuan Fu*, Zhijie Chang*. Varp is a Rab21 guanine nucleotide exchange factor and regulates endosome dynamics. Journal of Cell Science. (co-corresponding authors). 119(Pt 6):1053-62, 2006.
　·Zhijie Chang and Jim Hu. RNAi therapeutics: Can siRNAs conquer SARS? News and Commentary. Gene Therapy Vol. 13. 871-872, 2006.
　·Yue Chen, Ying-Hua Li, Xi-Ping Chen, Li-Min Gong, Shu-Ping Zhang, Zhijie Chang, Xiu-Fang Zhang*, Xin-Yuan Fu*, Li Liu*. Point Mutation at Single Tyrosine Residue of Novel Oncogene NOK Abrogates Tumorigenesis in Nude Mice. Cancer Research. Vol. 65(23), 10838-10846, 2005.
　·Hong Xin, Xialian Xu, Linyu Li, Hongxiu Ning, Yu Rong, Yu Shang, Yinyin Wang, Xin-Yuan Fu, and Zhijie Chang*. CHIP controls the sensitivity of TGF-beta signaling by maintaining the basal level of Smad3 through ubiquitin-mediated degradation. J. Biol. Chem. Vol. 280, No. 21, 20842-20850, 2005.
　·Tieshi Li, Yuanjiang Zhang, Ling Fu, Changming Yu, Xueni Li, Yinghua Li, Xinjun Zhang, Zhili Rong, Yinyin Wang, Hongxiu Ning, Rong Liang, Wei Chen, Lorne A. Babiuk, and Zhijie Chang*. siRNA targeting Leader sequence of SARS-CoV inhibited the virus replication. Gene Therapy Vol. 12, 751-761, 2005.
　·Li Liu, Xin-Zi Yu, Tie-Shi Li, Lian-Xia Song, Pei-La Chen, Ta-Lin Suo, Ying-Hua Li, Shi-Dong Wang, Yue Chen, Yong-Ming Ren, Shu-Ping Zhang, Zhi-Jie Chang, Xin-Yuan Fu*. A Novel Protein Tyrosine Kinase NOK that Shares Homology with Platelet-Derived Growth Factor/Fibroblast Growth Factor Receptors Induces Tumorigenesis and Metastasis in Nude Mice. Cancer Research. 64, 3491-3499, 2004.
　·Yuanjiang Zhang, Tieshi Li, Ling Fu, Changming Yu, Yinghua Li, Xialian Xu, Yinyin Wang, Hongxiu Ning, Shuping Zhang, Wei Chen, Lorne A Babiuk, and Zhijie Chang*. Silencing SARS-CoV Spike protein expression in cultured cells by RNA interference. FEBS Letters Vol. 560, 1-3 pp141-146, 2004.
　·Keiko Tamai, Xin Zeng, Chunming Liu, Xinjun Zhang, Yuko Harada, Zhijie Chang, and Xi He. A mechanism for Wnt co-receptor activation. Molecular Cell Vol. 13, 149-156, Jan. 16, 2004.
　·Linyu Li, Hong Xin, Xialian Xu, Mei Huang, Xinjun Zhang, Yue Chen, Shuping Zhang, Xin-Yuan Fu* and Zhijie Chang*. CHIP mediates degradation of Smad proteins and potentially regulates Smad-induced transcription. Mol. Cell. Biol. Vol. 24, No. 2, p856-864, 2004. (co-corresponding authors)
　·Shiqin Xiong, Qiuhui Zhao, Zhili Rong, Guanrong Huang, Yiling Huang, Peila Chen, Shuping Zhang, Li Liu, and Zhijie Chang*. hSef inhibits PC12 cell differentiation by interfering with Ras-Mitogen-activated MAPK signaling. J. Biol. Chem. Vol. 278, No.50, 50273-50282, 2003. 
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E-mail: zhijiec@tsinghua.edu.cn 
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